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Sparse feature selection methods identify unexpected global cellular response to strontium-containing materials.

Hélène Autefage1, Eileen Gentleman2, Elena Littmann1

  • 1Departments of Materials and Bioengineering and Institute of Biomedical Engineering, Imperial College London, London SW7 2AZ, United Kingdom;

Proceedings of the National Academy of Sciences of the United States of America
|April 2, 2015
PubMed
Summary
This summary is machine-generated.

Strontium-substituted bioactive glass (BG) alters human mesenchymal stem cell metabolism, up-regulating the isoprenoid pathway. This biomaterial modification impacts cellular cholesterol and lipid raft content, offering new insights into cell-material interactions.

Keywords:
human mesenchymal stem cellsmevalonate pathwaymicroarray analysissparse feature selection analysisstrontium-releasing biomaterials

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Area of Science:

  • Biomaterials Science
  • Cellular Biology
  • Biochemistry

Background:

  • Understanding cellular global responses to biomaterials is crucial for advanced design.
  • Current characterization methods often lack a holistic view of cell-material interactions.

Purpose of the Study:

  • To investigate the global response of human mesenchymal stem cells (hMSCs) to strontium-substituted 45S5 bioactive glass (BG).
  • To explore how strontium incorporation influences cellular pathways and composition.

Main Methods:

  • Nontargeted holistic biological and physical science techniques.
  • Whole gene-expression profiling, Raman spectroscopy mapping, and total internal reflection fluorescence microscopy.
  • Standard molecular biology techniques for confirmation.

Main Results:

  • Strontium-substituted BG significantly up-regulated the isoprenoid pathway in hMSCs.
  • Increased cellular and membrane cholesterol and lipid raft content were observed.
  • Elevated levels of phosphorylated myosin II light chain were detected.

Conclusions:

  • Biomaterial composition, specifically strontium ion incorporation, can strongly regulate cellular metabolic pathways.
  • Discovery-driven, nonreductionist approaches are beneficial for understanding cell-material interactions.
  • Findings suggest novel strategies for biomaterial evaluation and design.