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Isolation and Identification of Bacterial Strains from Skin of Terrestrial Amphibians
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Citrobacter bitternis sp. nov. isolated from bitterns
Kwan Soo Ko1, Ji-Young Choi, Joo Kim
1Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Korea, ksko@skku.edu.
Current Microbiology
|April 3, 2015
Summary
Two novel gram-negative bacteria, Citrobacter bitterns sp. nov., were identified from bitterns. These bacteria represent a new species within the Citrobacter genus, distinguished by unique biochemical and genotypic characteristics.
Area of Science:
- Microbiology
- Bacteriology
- Genomics
Background:
- The genus Citrobacter comprises several clinically relevant species.
- Bacterial diversity in avian habitats remains an area for exploration.
Purpose of the Study:
- To characterize and classify two novel gram-negative bacterial isolates from bitterns.
- To determine if these isolates represent a new species within the Citrobacter genus.
Main Methods:
- 16S rRNA gene sequencing for phylogenetic analysis.
- Cellular fatty acid composition analysis.
- Biochemical tests for metabolic profiling.
Main Results:
- Two strains, SKKU-TP7(T) and SKKU-TP20, were isolated from bitterns.
- Phylogenetic analysis based on 16S rRNA gene sequences showed close relatedness to Citrobacter farmeri.
- Distinct cellular fatty acid profiles and biochemical characteristics differentiated the isolates from known Citrobacter species.
Conclusions:
- The data support the classification of strains SKKU-TP7(T) and SKKU-TP20 as a novel species, Citrobacter bitterns sp. nov.
- The proposed type strain is SKKU-TP7(T).
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