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Updated: Apr 15, 2026

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
Published on: June 13, 2016
Strategies for isolation of exosomes
Emily Zeringer1, Timothy Barta1, Mu Li1
1Thermo Fisher Scientific, Austin, Texas 78744.
Abstract:
Exosomes are tiny vesicles (diameter 30-150 nm) secreted by cells in culture and found in all body fluids. These vesicles, loaded with unique RNA and protein cargos, have many biological functions, of which only a small fraction is currently understood-for example, they participate in cell-to-cell communication and signaling within the human body. The spectrum of current scientific interest in exosomes is wide and ranges from understanding their functions and pathways to using them in diagnostics, as biomarkers, and in the development of therapeutics. Here we provide an overview of different strategies for isolation of exosomes from cell-culture media and body fluids.
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