[Lymphocyte subpopulations in the bone marrow of children]

Blood lymphocyte subpopulations, and bone marrow aspiration and biopsy materials were comparatively investigated in 10 children without hematologic or infectious diseases (the children had chest deformations and diaphragmatic hernias). Lymphocyte typing was conducted by the method of continuous cytofluorometry in a complete leucocyte suspension with the use of monoclonal antibodies. The results of the study have evidenced that the content of lymphocytes carrying T-cell markers in the aspiration material was twofold lower, and in the biopsy material fourfold lower than in the peripheral blood. The number of immature T-lymphocytes in the bone marrow is higher than their content in the peripheral blood. A total of 40% of aspiration cells and 60% of biopsy cells present in the lymphocytic window, do not carry lymphocytic markers. CD8+-lymphocytes prevail in the bone marrow.