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Updated: Jul 31, 2026

Isolation of Human Neutrophils from Whole Blood and Buffy Coats
Published on: September 17, 2021
[Subpopulations of peripheral blood neutrophils and lymphocytes in children with immune neutropenia]
Abstract:
Peripheral blood (PB) neutrophils and lymphocytes from 14 patients with immune neutropenia were studied using the technique of rosette-formation in 2 neutrophil fractions with diverse specific density. All the neutropenia cases were divided into 2 groups by phagocytosis completeness in all the PB neutrophil types studied. Patients of group 1 had impaired phagocytosis D-RFN in elevated levels of these cells, autorosette-forming lymphocytes and neutrophils. Patients of group 2 had defective phagocytosis D-RFN and EAC-RFN in combination with extremely high contents of autorosette-forming including early lymphocytes. The above complex of techniques may be used for prediction of the duration and severity of neutropenia which is important for further selective studies into the causes of neutropenia and effective treatment.
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