Method for fluorescent marker swapping and its application in Steinernema nematode colonization studies
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Samuel Gengler1, Henri Batoko2, Pierre Wattiau3
1Veterinary & Agrochemical Research Centre, Brussels, Belgium; Institute of life sciences (ISV), Catholic University of Louvain-la-Neuve (UCL), Belgium.
Journal of Microbiological Methods
|April 4, 2015
Abstract:
An allelic exchange vector was constructed to replace gfp by mCherry in bacteria previously tagged with mini-Tn5 derivatives. The method was successfully applied to a gfp-labeled Yersinia pseudotuberculosis strain and the re-engineered bacterium was used to study the colonization of Steinernema nematodes hosting their Xenorhabdus symbiont using dual-color confocal microscopy.
