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Alterations in electrolyte and trace-element concentrations during simulated extracorporeal blood circulation
W Bergmann1, W Heller, H E Hoffmeister
1Chirurgische Universitätsklinik, Tübingen, W. Germany.
Free Radical Research Communications
|January 1, 1989
Summary
Simulated extracorporeal blood circulation damages blood cells, particularly with oxygen. Oxygen gas flow alters plasma electrolyte and trace-element levels, indicating induced membrane permeability.
Area of Science:
- Biomedical Engineering
- Hematology
- Clinical Chemistry
Background:
- Extracorporeal blood circulation (ECC) is vital in medical procedures.
- Potential adverse effects of ECC on blood components require thorough investigation.
- Understanding plasma chemistry changes during ECC is crucial for patient safety.
Purpose of the Study:
- To investigate the damaging effects of simulated ECC on blood cells.
- To analyze electrolyte and trace-element concentration changes in plasma during simulated ECC.
- To determine the primary causes of cellular damage during ECC.
Main Methods:
- Simulated extracorporeal blood circulation model.
- Analysis of blood cell morphology and viability.
- Measurement of plasma electrolyte (e.g., magnesium, calcium) and trace-element (e.g., copper, iron) concentrations.
- Assessment of membrane permeability.
Main Results:
- Significant damaging effects on blood cells were observed, especially under oxygenating conditions.
- Oxygen gas flow led to the highest magnesium resorption and calcium, copper, and iron desorption.
- Induced membrane permeability for electrolytes was evident.
- Cellular damage attributed solely to mechanical stress was found to be negligible.
Conclusions:
- Oxygenation during simulated ECC significantly impacts blood cells and plasma composition.
- Alterations in electrolyte and trace-element levels suggest oxygen-induced membrane damage.
- Mechanical stress is not the primary driver of cellular damage in this perfusion system.