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In vivo induction of T-follicular helper cells by modulation of regulatory T cell function

James Badger Wing1, Shimon Sakaguchi

  • 1Laboratory of Experimental Immunology, WPI Immunology Frontier Research Center, Osaka University, 3-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|April 4, 2015
PubMed

Regulatory T cells, particularly their follicular resident subset, T-follicular regulatory cells, play a critical role in the maintenance of immune homeostasis and prevention of the expansion of T-follicular helper cells. One of the key mechanisms used by these cells is CTLA-4 dependent suppression of inflammation. In this chapter, we detail methods to increase the formation of T-follicular helper cells following vaccination by either depleting Treg cells, via Foxp3 linked diphtheria toxin receptor, or blocking CTLA-4 function by the use of antibodies.

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