Related Experiment Video
Updated: Jun 28, 2026
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
Published on: June 26, 2018
Protocol for live cell barcoding and immunophenotyping of human hematological malignancies using cytometry by time of
Takashi Mikami1, James Badger Wing2, Junko Takita3
1Division of Cancer Immune Multicellular System Regulation, Center for Cancer Immunotherapy and Immunobiology (CCII), Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.
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High-dimensional cell profiling by cytometry by time of flight (CyTOF) enables simultaneous characterization of rare cell populations but requires many antibodies and is sensitive to staining variability. Here, we present a workflow for immunophenotyping human hematological malignancy samples, reducing costs and minimizing technical variation. We describe steps for sample collection, live-cell barcoding using anti-β2-microglobulin and CD147 antibodies, cell staining, data collection, and subsequent analyses. For complete details on the use and execution of this protocol, please refer to Mikami et al.1.
