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Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes
Published on: April 9, 2015
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Cryopreservation of neonatal cardiomyocytes
Adam C Vandergriff1, M Taylor Hensley, Ke Cheng
1Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, 27607, USA.
Methods in Molecular Biology (Clifton, N.J.)
|April 4, 2015
Abstract:
Cardiomyocytes are frequently used for in vitro models for cardiac research. The isolation of cells is time-consuming and, due to the cells limited proliferative abilities, must be performed frequently. To reduce the time requirements and the impact on research animals, we describe a method for cryopreserving neonatal rat cardiomyocytes (NRCMs), and subsequently thawing them for use in assays.

