Evaluation of sarcomeric organization in human pluripotent stem cell-derived cardiomyocytes

High-resolution optical imaging provides valuable window in examining transitional and systemic changes in cellular processes. The relative spatial relationship of structural, transport, and signaling proteins, surface antigens, and transcription factors may reveal developmental state of the cellular system. Here, we describe the use of confocal microscopy to evaluate the organization of sarcomeric structural proteins, sarcoplasmic channel proteins, and cardiac transcription factors in human pluripotent stem cell (PSC)-derived cardiomyocytes.