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Updated: Apr 15, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
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Inducing gene expression by targeting promoter sequences using small activating RNAs.

Ji Wang1, Robert F Place1, Victoria Portnoy1

  • 1Department of Urology and Helen-Diller Comprehensive Cancer Center, University of California San Francisco, San Francisco, CA 94158, USA.

Journal of Biological Methods
|April 4, 2015
PubMed
Summary

Small activating RNAs (saRNAs) offer a novel method to activate endogenous gene expression by targeting promoter regions. This RNA activation (RNAa) technique provides an alternative to traditional vector systems for gene studies and cell reprogramming.

Keywords:
RNAagene regulationsaRNAtranscriptional activation

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Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Epigenetics

Background:

  • Traditional vector-based systems for gene expression have limitations, including complex construction, exclusion of crucial regulatory sequences, and potential for insertional mutagenesis.
  • Exogenous nucleic acid delivery systems are standard for ectopic gene expression but present significant drawbacks.

Purpose of the Study:

  • To present a detailed protocol for utilizing RNA activation (RNAa) for endogenous gene expression.
  • To describe methods for small activating RNA (saRNA) design and target selection for RNAa.
  • To facilitate the application of RNAa in cultured cells for gene function studies.

Main Methods:

  • Design of double-stranded RNAs (dsRNAs) targeting specific promoter sequences.
  • Application of dsRNAs to cultured cells to induce endogenous gene expression via RNA activation.
  • Experimental validation of RNAa-induced gene expression.

Main Results:

  • Demonstration of a protocol for effective RNA activation (RNAa) in cultured cells.
  • Successful induction of endogenous gene expression using small activating RNAs (saRNAs).
  • Validation of saRNA design and target selection strategies for RNAa.

Conclusions:

  • RNA activation (RNAa) mediated by small activating RNAs (saRNAs) provides a powerful tool for endogenous gene modulation.
  • This technique offers an alternative to conventional gene delivery methods, overcoming limitations associated with vector systems.
  • RNAa holds potential for diverse applications, including gene function analysis, pathway interrogation, and cell fate reprogramming.