Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

Cell Specific Gene Expression

Cell Specific Gene Expression

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Special Issue on Mammalian Cell Synthetic Biology.

ACS synthetic biology·2026

Same author

Engineering non-ribosomal peptide synthesis: tuning the antibiotics engine of the microbial world.

Critical reviews in biotechnology·2026

Same author

What problem do you hope bioengineering or synthetic biology approaches will enable us to tackle in the next decade?

Cell systems·2026

Same author

Slowpoke: An Automated Golden Gate Cloning Workflow for Opentrons OT-2 and Flex.

ACS synthetic biology·2026

Same author

Artificial Symbiosis for Bulk Production of Bacterial Cellulose Composites.

Advanced materials (Deerfield Beach, Fla.)·2026

Same author

Programmable cell-cell adhesion in synthetic yeast communities for improved bioproduction.

Nature chemical biology·2026

Same journal

Efficient evidence-based genome annotation with EviAnn.

Nature methods·2026

Same journal

ClairS: a deep-learning method for long-read tumor-normal pair somatic small variant calling.

Nature methods·2026

Same journal

RNAbpFlow: base pair-augmented SE(3) flow matching for conditional RNA 3D structure generation.

Nature methods·2026

Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026

Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026

Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 15, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Quantifying cellular capacity identifies gene expression designs with reduced burden.

Francesca Ceroni¹, Rhys Algar¹, Guy-Bart Stan¹

¹1] Centre for Synthetic Biology and Innovation, Imperial College London, London, UK. [2] Department of Bioengineering, Imperial College London, London, UK.

|April 8, 2015

Summary

This summary is machine-generated.

We developed a new method to monitor cellular burden in real time. This tool helps identify synthetic gene construct designs that minimize cellular stress and improve efficiency in Escherichia coli.

More Related Videos

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Published on: February 25, 2017

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

Related Experiment Videos

Last Updated: Apr 15, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Published on: February 25, 2017

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

Area of Science:

Synthetic biology
Microbial engineering
Cellular physiology

Background:

Heterologous gene expression imposes a metabolic burden on host cells.
Quantifying this burden is crucial for designing efficient synthetic biological systems.

Purpose of the Study:

To develop an in vivo monitor for real-time assessment of cellular capacity.
To assay the burden imposed by synthetic constructs in Escherichia coli.
To identify construct designs that minimize cellular burden.

Main Methods:

Development of a novel in vivo monitoring system for Escherichia coli.
Real-time tracking of cellular capacity changes.
Assay of burden from synthetic constructs and their components.

Main Results:

Identified specific construct designs that significantly reduce cellular burden.
Demonstrated predictable performance improvements in reduced-burden designs.
Showcased designs with equivalent output but lower cellular cost.

Conclusions:

The in vivo monitor effectively quantifies cellular burden from gene expression.
Optimized construct design can mitigate the negative impacts of heterologous gene expression.
This approach facilitates the development of more efficient synthetic biological systems.