Quantifying cellular capacity identifies gene expression designs with reduced burden
Francesca Ceroni1, Rhys Algar1, Guy-Bart Stan1
11] Centre for Synthetic Biology and Innovation, Imperial College London, London, UK. [2] Department of Bioengineering, Imperial College London, London, UK.
Nature Methods
|April 8, 2015
Abstract:
Heterologous gene expression can be a significant burden for cells. Here we describe an in vivo monitor that tracks changes in the capacity of Escherichia coli in real time and can be used to assay the burden imposed by synthetic constructs and their parts. We identify construct designs with reduced burden that predictably outperformed less efficient designs, despite having equivalent output.
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