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Updated: Apr 15, 2026
A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells
Published on: March 3, 2016
Activin A can induce definitive endoderm differentiation from human parthenogenetic embryonic stem cells
Zhiqiang Wang1, Wenliang Li, Tianxing Chen
1Department of Oncology, The First Affiliated Hospital of Kunming Medical University, Kunming, 650032, People's Republic of China.
Objectives:
As activin/nodal signaling plays a key role in definitive endoderm (DE) differentiation, we have explored activin A-induced differentiation of DE from human parthenogenetic embryonic stem cells (hPESCs).
Results:
Administration of 5 ng activin A/ml had no effect on the expression of markers of DE differentiation. However, higher concentrations of activin A (50 and 100 ng/ml) upregulated Sox17 and Cxcr4, as well upregulating the mesendodermal precursor marker, Brachyury.
Conclusions:
These findings demonstrate that low dose activin A can maintain the undifferentiated potency of hPESCs, whereas higher doses induce DE differentiation; 50 ng/ml is the optimal concentration for inducing DE from hPESCs.
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