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Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
Published on: March 29, 2018
[The effect of fluoride on the expression of GRP-78 and caspase-12 in rat ameloblast]
1Department of Preventive Dentistry, Central Laboratory, School of Stomatology, China Medical University. Shenyang 110002, Liaoning Province, China.E-mail:794239430@qq.com.
Purpose:
To study the effect of GRP-78 and caspase-12 on fluoride-induced endoplasmic reticulum stress and apoptosis in rat ameloblast, and explore whether fluoride-induced endoplasmic reticulum stress results in the occurrence of apoptosis.
Methods:
The cell activity of ameloblast cultured in various concentrations of fluoride was measured by CCK8 and flow cytometry; Real-time RT-PCR and Western blot were used to analyze the endoplasmic reticulum chaperone GRP-78 and caspase-12 genes and the expression of related proteins. The data was analyzed with SPSS13.0 software package.
Results:
With the increasing concentration of fluoride, the cell activity of rat ameloblasts decreased gradually, and flow cytometry also showed that the number of apoptosis gradually increased; Real-time RT-PCR and Western blot showed the expression of GRP-78 and caspase-12 increased while the fluoride concentration increased.
Conclusions:
Excessive fluoride induces endoplasmic reticulum stress of rat ameloblast, and leads to cell apoptosis.
