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Microfluidic Chip Fabrication and Method to Detect Influenza
Published on: March 26, 2013
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Engineered self-assembling monolayers for label free detection of influenza nucleoprotein
Anton P Le Brun1, Andrei Soliakov, Deepan S H Shah
1Bragg Institute, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, NSW, 2232, Australia.
Biomedical Microdevices
|April 11, 2015
Summary
Researchers developed a novel bio-interface using engineered proteins to create a sensor for detecting influenza nucleoprotein. This self-assembled monolayer on microelectronic devices offers a new method for label-free biosensing applications.
Area of Science:
- Nanotechnology
- Biosensing
- Surface Science
Background:
- Integrating nanotechnology into devices requires advanced methods for in situ analysis.
- Developing reliable biosensors necessitates precise control over molecular assembly and detection.
Purpose of the Study:
- To engineer a self-assembling protein monolayer for oriented antibody display on microelectronic devices.
- To develop a label-free sensor for influenza nucleoprotein detection.
- To characterize the bio-interface and its sensing capabilities.
Main Methods:
- Utilized engineered protein chimeras for self-assembling monolayers on gold surfaces.
- Employed surface plasmon resonance, quartz-crystal microbalance, and neutron reflectometry for characterization.
- Demonstrated label-free detection of influenza nucleoprotein.
Main Results:
- Engineered a 13.5 nm thick monolayer that self-assembles on gold.
- Achieved high-affinity capture of immunoglobulin G (IgG) in a defined orientation.
- Confirmed specific recognition of influenza A nucleoprotein.
- Showcased irreversible binding of IgG via in situ chemical crosslinking without functional impairment.
Conclusions:
- The developed protein-based bio-interface enables precise control over molecular assembly for biosensing.
- This strategy facilitates the transition from nanoscale engineered molecules to functional biosensor arrays.
- The method provides a detailed understanding of bio-interface formation and its application in influenza detection.
