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Updated: Apr 15, 2026

Detection of Foodborne Bacterial Pathogens from Individual Filth Flies
Published on: February 13, 2015
Pulsed-field gel electrophoresis (PFGE) for pathogenic Cronobacter species
Qiongqiong Yan1, Séamus Fanning
1UCD-Centre for Food Safety, WHO Collaborating Centre for Research, Reference and Training on Cronobacter, School of Public Health, Physiotherapy and Population Science, University College Dublin, Belfield, Dublin 4, Ireland.
Abstract:
Pulsed-field gel electrophoresis (PFGE) is a molecular-based subtyping strategy that uses a suitable DNA restriction endonuclease enzyme to cut genomic DNA into several large linear fragments, that can be separated based on their sizes. PFGE has been successfully applied to the subtyping of many pathogenic bacteria, including Cronobacter species, and it is commonly considered as a "gold standard" in epidemiological studies.
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