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[Identification of proteins interacting with the circadian clock protein PER1 in tumors using bacterial two-hybrid
Yu Zhang1, Youlin Yao, Siyuan Jiang
1Department of Medical Genetics and Division of Morbid Genomics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China. mayongxin@gmail.com.
Objective:
To identify protein-protein interaction partners of PER1 (period circadian protein homolog 1), key component of the molecular oscillation system of the circadian rhythm in tumors using bacterial two-hybrid system technique.
Methods:
Human cervical carcinoma cell Hela library was adopted. Recombinant bait plasmid pBT-PER1 and pTRG cDNA plasmid library were cotransformed into the two-hybrid system reporter strain cultured in a special selective medium. Target clones were screened. After isolating the positive clones, the target clones were sequenced and analyzed.
Results:
Fourteen protein coding genes were identified, 4 of which were found to contain whole coding regions of genes, which included optic atrophy 3 protein (OPA3) associated with mitochondrial dynamics and homo sapiens cutA divalent cation tolerance homolog of E. coli (CUTA) associated with copper metabolism. There were also cellular events related proteins and proteins which are involved in biochemical reaction and signal transduction-related proteins.
Conclusion:
Identification of potential interacting proteins with PER1 in tumors may provide us new insights into the functions of the circadian clock protein PER1 during tumorigenesis.
Insights
Researchers identified novel protein partners interacting with the circadian clock protein PER1 in tumors. This discovery offers new insights into PER1
Area of Science:
- Molecular Biology
- Cancer Research
- Circadian Biology
Background:
- The circadian rhythm is regulated by molecular oscillations.
- PER1 (period circadian protein homolog 1) is a key component of these oscillations.
- Understanding PER1's interactions is crucial for cancer research.
Purpose of the Study:
- To identify protein-protein interaction partners of PER1 in tumors.
- To utilize the bacterial two-hybrid system for this identification.
Main Methods:
- A human cervical carcinoma cell Hela library was used.
- Recombinant plasmids (pBT-PER1 and pTRG cDNA library) were co-transformed into a reporter strain.
- Screening, isolation, sequencing, and analysis of target clones were performed.
Main Results:
- Fourteen protein coding genes interacting with PER1 were identified.
- Four genes contained whole coding regions, including OPA3 (mitochondrial dynamics) and CUTA (copper metabolism).
- Other identified proteins are involved in cellular events, biochemical reactions, and signal transduction.
Conclusions:
- This study identified potential interacting proteins with PER1 in tumors.
- These findings may offer new insights into the role of PER1 in tumorigenesis.
- Further research can explore the functional significance of these interactions in cancer development.
