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Related Experiment Video

Updated: Apr 15, 2026

Determination of Inorganic Arsenic in a Wide Range of Food Matrices using Hydride Generation - Atomic Absorption Spectrometry.

Determination of Inorganic Arsenic in a Wide Range of Food Matrices using Hydride Generation - Atomic Absorption Spectrometry.

Published on: September 1, 2017

Arsenic extraction and speciation in plants: Method comparison and development.

Di Zhao¹, Hong-Bo Li¹, Jia-Yi Xu¹

¹State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046, PR China.

The Science of the Total Environment

|April 13, 2015

Summary

This summary is machine-generated.

A new ethanol/water extraction method efficiently isolates arsenic (As) from plants for speciation analysis. This optimized technique offers higher extraction yields and preserves arsenic species, proving effective across various plant types.

Keywords:

Extraction method HPLC–ICP-MS Hyperaccumulator Pteris vittata Speciation

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Area of Science:

Environmental Chemistry
Plant Science
Analytical Chemistry

Background:

Arsenic (As) speciation is crucial for understanding its toxicity and bioavailability in plants.
Existing extraction methods can alter As species, complicating accurate speciation analysis.
Efficient extraction is needed for diverse plant matrices with varying As concentrations.

Purpose of the Study:

To compare four methods for arsenic extraction from three plant species for speciation analysis.
To develop a more efficient and reliable As extraction method.
To optimize extraction parameters for improved efficiency and species preservation.

Main Methods:

Compared heating with dilute HNO3, sonication with phosphate buffered solution, methanol/water, and ethanol/water for As extraction.
Analyzed As species using high-performance liquid chromatography coupled with inductively-coupled plasma mass spectrometry (HPLC-ICP-MS).
Optimized the ethanol/water method by adjusting extraction time, ethanol concentration, and sample:solution ratio.

Main Results:

The ethanol/water method demonstrated superior extraction efficiency (~80% roots, >85% fronds in Pteris vittata) without altering As species.
Optimized ethanol/water method used 25% ethanol and shorter sonication times (0.5h fronds, 2h roots).
Satisfactory extraction yields (78-92% tobacco, ~70% rice) were achieved with the optimized method across different plant types.

Conclusions:

The optimized ethanol/water method is highly efficient for arsenic extraction and speciation in Pteris vittata, rice, and tobacco.
This method minimizes changes to arsenic species during extraction, ensuring reliable speciation analysis.
The developed method shows potential for broad application in As speciation across various plant species.