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Expanding the Utility of FUCCI Reporters Using FACS-Based Omics Analysis
James Chappell1, Ben Boward1, Stephen Dalton2,3
1Department of Biochemistry and Molecular Biology, University of Georgia, 500 DW Brooks Drive, Athens, GA, 30602, USA.
Abstract:
The FUCCI indicator system is a powerful tool for spatio-temporal analysis of the cell cycle, but its utility has been restricted so far to a limited range of applications. Here, we describe how to establish and validate the FUCCI system in murine pluripotent stem cells (PSCs) and describe the utility of transgenic FUCCI mice. We then describe how the FUCCI system can be used to generate material for a wide-range of omics-based applications in conjunction with FACS isolation of cells. This significantly broadens the potential applications of FUCCI reporters for studying the molecular basis of development and disease.
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