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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Establishing an RNA Sensor with High Sensitivity and Dynamic Range Utilizing a Signal Amplifier Platform.

Ha Eun Lim¹, Carlos D Llanos^1,2, James Chappell^1,2,3

¹Department of Bioengineering, Rice University, Houston 77005Texas, United States.

ACS Synthetic Biology

|March 27, 2026

Summary

This summary is machine-generated.

This study enhances RNA sensors (RADAR) with signal amplification for precise gene expression control. The improved platform detects low-abundance transcripts, aiding precision therapeutics in dynamic disease environments.

Keywords:

RADAR sensors RNA sensors gene signal amplifier hypoxia response mammalian synthetic biology unfolded protein response

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Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

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Area of Science:

Molecular Biology
Biotechnology
Genetics

Background:

Precise gene expression control is vital for treating dynamic diseases.
Current methods struggle with heterogeneous cell states in conditions like cancer and inflammation.
RNA sensors offer programmable cell state classification but have sensitivity limitations.

Purpose of the Study:

To enhance the sensitivity and dynamic range of RNA sensing platforms.
To develop a tool for real-time monitoring of endogenous transcript abundance.
To enable precision therapeutic strategies in complex disease microenvironments.

Main Methods:

Integration of RADAR (RNA sensing using Adenosine Deaminases Acting on RNA) sensors with a signal amplification circuit.
Development of a combined RADAR-amplifier platform.
Testing the platform's ability to monitor endogenous transcript changes.

Main Results:

The RADAR-amplifier platform significantly enhanced sensitivity and dynamic range.
The system enables real-time monitoring of subtle endogenous transcript abundance changes.
Demonstrated utility in physiological conditions.

Conclusions:

The enhanced RADAR-amplifier platform overcomes limitations of traditional RNA sensors.
This technology is valuable for fundamental biological research.
It holds promise for developing advanced precision therapeutic strategies.