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Related Experiment Video

Updated: Jan 16, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Establishing an RNA sensor with high sensitivity and dynamic range utilizing a signal amplifier platform.

Ha Eun Lim¹, James Chappell^1,2, Laura Segatori^1,2,3

¹Department of Bioengineering, Rice University, Houston, TX, 77005, USA.

Biorxiv : the Preprint Server for Biology

|September 26, 2025

Summary

This study enhances RNA sensors (RADAR) with signal amplification for precise gene expression monitoring. The improved platform offers higher sensitivity and dynamic range for detecting low-abundance transcripts in dynamic disease environments.

Keywords:

RADAR sensors RNA sensors gene signal amplifier mammalian synthetic biology

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Last Updated: Jan 16, 2026

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Published on: July 8, 2025

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Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

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Published on: September 20, 2020

Area of Science:

Biotechnology
Molecular Biology
Gene Expression Regulation

Background:

Precise gene expression control is crucial for treating dynamic diseases like cancer and inflammation.
Current methods struggle with heterogeneous cell states and low-abundance transcripts.
RNA sensors offer programmable cell-state classification but have limited sensitivity.

Purpose of the Study:

To enhance the sensitivity and dynamic range of RNA sensors for detecting endogenous transcripts.
To develop a platform for real-time monitoring of gene expression in complex biological systems.
To enable precision therapeutic strategies through improved transcript detection.

Main Methods:

Integration of RADAR (RNA sensing using Adenosine Deaminases Acting on RNA) sensors with a signal amplification circuit.
Development of a combined RADAR-amplifier platform for transcript detection.
Testing the platform's performance under physiological conditions for real-time monitoring.

Main Results:

The integrated RADAR-amplifier platform significantly enhanced sensitivity and dynamic range.
The system enabled real-time monitoring of subtle changes in endogenous transcript abundance.
Demonstrated utility for fundamental biological studies and precision therapeutics.

Conclusions:

The enhanced RADAR-amplifier platform overcomes limitations of traditional RNA sensors.
This technology provides a sensitive tool for studying gene expression in dynamic environments.
The platform holds promise for advancing precision medicine and therapeutic development.