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Updated: Apr 15, 2026
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Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis
Published on: November 17, 2020
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Methods for Ex Vivo Analysis of Immune Cell Function from the Central Nervous System
Darryl G Turner1, Melanie D Leech, Richard A O'Connor
1MRC Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.
Methods in Molecular Biology (Clifton, N.J.)
|April 13, 2015
Summary
This protocol details processing immune tissues from mice with experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis. It enables analysis of immune cell function and phenotype in the central nervous system and peripheral organs.
Area of Science:
- Neuroimmunology
- Autoimmunity
- Animal models of disease
Background:
- Experimental autoimmune encephalomyelitis (EAE) is a key animal model for studying organ-specific autoimmunity.
- EAE serves as a model for the early immune responses observed in multiple sclerosis (MS).
Purpose of the Study:
- To outline a protocol for processing immune tissues in murine EAE.
- To facilitate the analysis of immune cell phenotype and function during EAE.
Main Methods:
- Processing of peripheral immune tissues: spleen and draining lymph nodes.
- Processing of the central nervous system (CNS) as the site of inflammation.
- Analysis of immune cell phenotype and function.
Main Results:
- The protocol enables comprehensive analysis of immune responses in EAE.
- It allows for detailed examination of immune cells in both CNS and peripheral tissues.
Conclusions:
- This standardized protocol supports research into EAE and its relevance to multiple sclerosis.
- It provides a framework for investigating immune cell dynamics in autoimmune CNS inflammation.
