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Updated: Apr 15, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
Published on: May 30, 2012
Maintenance, Transgene Delivery, and Pluripotency Measurement of Mouse Embryonic Stem Cells
1Department of Biological Sciences, Chemical and Biomolecular Engineering, University of Notre Dame, 49 Galvin Life Sciences, Notre Dame, IN, 46556, USA. Tetsuya.Tanaka.9@nd.edu.
Abstract:
This chapter describes standard techniques to (1) maintain mouse embryonic stem cell culture, (2) deliver transgenes into mouse embryonic stem cells mediated by electroporation, nucleofection, lipofection, and retro/lentiviruses, and (3) assess the pluripotency of mouse embryonic stem cells. The last part of this chapter presents induction of random cell differentiation followed by the alkaline phosphatase and embryoid body formation assays, immunofluorescence microscopy, and the teratoma formation assay.

