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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
A plasmonic aptasensor for ultrasensitive detection of thrombin via arrested rolling circle amplification
Sai Wang1, Sai Bi, Zonghua Wang
1College of Chemical Science and Engineering, Laboratory of Fiber Materials and Modern Textiles, The Growing Base for State Key Laboratory, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao 266071, P. R. China. bisai11@126.com wangzonghua@qdu.edu.cn.
Abstract:
A sensitive signal generation mechanism for gold nanoparticle growth by reducing gold ions with hydrogen peroxide is applied in a plasmonic aptasensor, achieving naked-eye detection of thrombin at the single-molecule level based on the specific interaction of aptamer-thrombin via an arrested rolling circle amplification to yield horseradish peroxidase (HRP)-mimicking DNAzymes as biocatalysts.
