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The effect of Oral Rehydration Solution on apoptosis of periodontal ligament cells
Seyed Ebrahim Jabarifar1, Seyed Mohammad Razavi2, Maryam Haje Norouzali Tehrani3
1Department of Pediatric Dentistry, khorasgan islamic azad university, Isfahan, Iran.
Background:
Over the years, numerous studies have been conducted to find a storage medium with favorable properties to preserve the avulsed teeth. Oral Rehydration Solution (ORS) is a simple oral solution containing glucose and electrolytes. In addition to physiologic PH and osmolality, it has other important properties such as availability and inexpensiveness, storing potential in different conditions and places, and long lifetime. This study was carried out to more precisely investigate the storage of cells in this substance in different concentrations and in longer times, and to evaluate the programmed cell death or apoptosis as well as cell viability.
Material And Methods:
The cultured PDL cells of this experimental study were exposed to three concentrations of 25%, 50%, and 100% ORS in comparison with Hanks Balanced Salt Solution (HBSS) and tap water. They were then analyzed by Tetrazolium Salt-based Colorimetric (MTT) Assay in 2-, 6-, 12-, 24-, and 48-h periods and by Apoptosis Assay in 12-, 24-, and 48-h time periods. Data were analyzed by spss software using anova and Post hoc (Duncan) tests. P ≤ 0.05 was considered significant.
Results:
Overall, the maximum viable cells and minimum apoptotic cells were reported after the cells' exposure to ORS (100%). Also, the minimum level of viable cells was observed in ORS (25%) group and the highest level of apoptotic cells was observed in 25% and 50% ORS groups.
Conclusion:
ORS preserved more viable cells and induced fewer apoptotic cells in comparison with HBSS.
