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Synthesis of silver nanoparticle and its application
A Muthu Kumara Pandian1, C Karthikeyan1, M Rajasimman1
1Environmental Engineering Laboratory, Department of Chemical Engineering, Annamalai University, Annamalai Nagar, Tamil Nadu, India.
Ecotoxicology and Environmental Safety
|April 14, 2015
Summary
Green synthesis yielded high amounts of silver nanoparticles, which were characterized and tested for cancer therapy. These nanoparticles showed cytotoxic effects on cancer cells, indicating potential for future treatments.
Area of Science:
- Nanotechnology
- Materials Science
- Biomedical Engineering
Background:
- Silver nanoparticles (AgNPs) are increasingly investigated for biomedical applications due to their unique properties.
- Various synthesis methods exist, but optimizing yield and biocompatibility is crucial for therapeutic use.
Purpose of the Study:
- To synthesize silver nanoparticles using wet chemical, green synthesis, and microbial methods.
- To characterize the synthesized AgNPs and evaluate their cytotoxic effects on cancerous and normal cell lines.
- To assess the potential of AgNPs as a therapeutic agent for cancer treatment.
Main Methods:
- Synthesis of AgNPs via wet chemical, green synthesis (using aqueous extract), and microbial methods.
- Characterization using Scanning Electron Microscopy (SEM) for size determination and Fourier Transform Infrared spectroscopy (FTIR) for biomolecule identification.
- Cytotoxicity assessment using MTT assay on cancerous and normal cell lines to determine optimal concentration and efficacy.
Main Results:
- Green synthesis demonstrated a higher yield of silver nanoparticles compared to other methods.
- SEM analysis provided size information of the synthesized AgNPs.
- FTIR confirmed the presence of biomolecules capping the AgNPs, suggesting a role in stabilization.
- MTT assay results indicated a dose-dependent cytotoxic effect of AgNPs on cancerous cell lines, with varying effects on normal cell lines.
Conclusions:
- Green synthesis is an effective method for high-yield silver nanoparticle production.
- Characterized silver nanoparticles exhibit cytotoxic properties against cancer cells, highlighting their therapeutic potential.
- Further research is warranted to optimize AgNP concentration and delivery for effective and safe cancer therapy.
