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Characterization of feline serum-cobalt binding
Amy N Schnelle1, Anne M Barger1, Amy L MacNeill1
1Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Champaign, IL, USA.
Veterinary Clinical Pathology
|April 14, 2015
Summary
Feline serum-cobalt binding, influenced by albumin, can now be measured with a new colorimetric test. Reduced binding in inflammation is linked to lower albumin levels.
Area of Science:
- Veterinary Biochemistry
- Clinical Pathology
Background:
- Oxidative stress impairs albumin's cobalt complexation ability.
- Feline serum-cobalt binding characteristics remain undescribed.
Purpose of the Study:
- Develop a novel colorimetric test for feline serum-cobalt binding.
- Correlate cobalt binding with feline blood constituents and clinical conditions.
Main Methods:
- A colorimetric assay utilizing cobalt (Co+2) and dithiothreitol was developed for feline serum.
- 176 feline serum samples were analyzed for cobalt binding, alongside hematology, biochemistry, and clinical data.
Main Results:
- Feline serum-cobalt binding showed a linear correlation with serial serum dilution (r² = .9984).
- Cobalt binding correlated with albumin concentration (r² = .425) and was decreased in inflammatory conditions.
- Albumin and cobalt binding correlated with erythron variables, total calcium, and bilirubin.
Conclusions:
- The developed test confirms feline albumin-cobalt complex formation.
- Hypoalbuminemia is identified as the probable cause of decreased serum-cobalt binding in feline inflammation.
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