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Related Experiment Video

Updated: Apr 15, 2026

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

A Collaborative Assembly Strategy for Tumor-Targeted siRNA Delivery.

Qiong Sun¹, Zisheng Kang¹, Lingjing Xue¹

¹State Key Laboratory of Natural Medicines and Jiangsu Key Laboratory of Drug Discovery for Metabolic Diseases, Center of Drug Discovery, China Pharmaceutical University, Nanjing 210009, China.

Journal of the American Chemical Society

|April 15, 2015

Summary

A novel collaborative assembly method enhances siRNA delivery systems, improving safety and efficiency. This approach creates stable, functionalized nanoparticles for targeted gene silencing.

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Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

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Published on: July 23, 2016

Area of Science:

Biomaterials Science
Nanotechnology
Gene Therapy

Background:

Conventional siRNA delivery systems often rely solely on physical assembly, limiting their safety, efficiency, and flexibility.
High cationic properties are crucial for lipid-based complexes to achieve substantial siRNA loading capacity.

Purpose of the Study:

To develop a novel "collaborative assembly" approach for synthesizing advanced siRNA delivery systems.
To improve the safety, efficiency, stability, and targeting capabilities of siRNA carriers.

Main Methods:

Combining electrostatically driven physical assembly with click reaction-mediated chemical assembly.
Chemically modifying a cationic lipid-polymer complex with hyaluronic acid (HA) via click chemistry.
Evaluating the in vivo stability, tumor targeting, and gene-silencing efficacy of the developed nanoparticles.

Main Results:

The collaborative assembly approach yielded a lipid-polymer hybrid nanoparticle (RSC-HA) with enhanced in vivo stability and tumor targeting compared to physically assembled systems.
Chemical modification via click chemistry shielded positive charges, reducing toxicity and improving blood stability without compromising siRNA binding.
The developed system demonstrated controlled siRNA release upon cellular uptake, leading to enhanced gene silencing.

Conclusions:

The collaborative assembly strategy offers a safe, stable, and functionalizable platform for siRNA delivery.
This hybrid approach overcomes limitations of purely physical assembly, paving the way for improved therapeutic applications.
Pre-functionalization of incorporated polyanions allows for tailored biological characteristics, such as prolonged circulation and tumor targeting.