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Published on: October 23, 2009
Electrochemical assay for determination of gluten in flour samples
Ece Eksin1, Gulsah Congur1, Arzum Erdem1
1Ege University, Faculty of Pharmacy, Analytical Chemistry Department, 35100, Bornova, Izmir, Turkey; Ege University, Graduate School of Natural and Applied Science, Biotechnology Department, 35100, Bornova, Izmir, Turkey.
Abstract:
Herein, an electrochemical assay developed by differential pulse voltammetry (DPV) in combination with the disposable pencil graphite electrode (PGE) was progressed for sensitive and selective detection of gluten. Under the optimum experimental conditions, a linear concentration range of gluten was obtained from 20 to 100 μg/mL and consequently the detection limit was found to be 7.11 μg/mL. In addition, this electrochemical assay was successfully employed for the detection of gluten in different flour samples. Applicability of our method was also tested in the commercial samples of vinegar and baker's yeast.
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