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Related Concept Videos

SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

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Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
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Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

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Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
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Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

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Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
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Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

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Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
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Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

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Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
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Neurons: The Cell Body and the Dendrites01:23

Neurons: The Cell Body and the Dendrites

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A typical nerve cell comprises three main components: the cell body, dendrites, and the axon. The cell body, also known as the soma or perikaryon, serves as the central biosynthetic hub housing a nucleus surrounded by cytoplasm containing organelles commonly found in most cells. Notably, Nissl bodies, clusters of the rough endoplasmic reticulum and free ribosomes responsible for protein synthesis, are distinctive features of the neuronal cell body. As neurons age, aggregates of a brown pigment...
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Related Experiment Video

Updated: Apr 14, 2026

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1&#946; in Human Monocyte-derived Dendritic Cells
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An Emerging Role for SNARE Proteins in Dendritic Cell Function.

Laura E Collins1, Joseph DeCourcey1, Mariana Soledad di Luca1

  • 1Immunomodulation Research Group, School of Biotechnology, Dublin City University , Dublin , Ireland.

Frontiers in Immunology
|April 16, 2015
PubMed
Summary

Soluble n-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins regulate cytokine secretion by dendritic cells (DCs), crucial for immune responses. Understanding SNAREs in DCs offers potential therapeutic targets for inflammatory diseases.

Keywords:
IL-12IL-23IL-6SNAREsdendritic cells

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Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface
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An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
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An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

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An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
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An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

Published on: April 18, 2016

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Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Dendritic cells (DCs) bridge innate and adaptive immunity by recognizing antigens via pattern-recognition receptors like Toll-like receptors (TLRs).
  • DCs secrete cytokines to manage homeostasis and pathogen clearance, but dysregulation can cause inflammatory diseases.
  • Cytokine secretion is tightly controlled by protein families involved in intracellular vesicle transport.

Purpose of the Study:

  • To review the role of soluble n-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins in dendritic cell (DC) function.
  • To highlight SNAREs as facilitators of cytokine secretion and tools for elucidating secretion pathways.
  • To explore SNAREs as potential therapeutic targets for inflammatory conditions.

Main Methods:

  • Review of literature on SNARE proteins and their function in cellular trafficking.
  • Analysis of SNARE localization within DCs to understand secretion pathways.
  • Discussion of SNARE specificity in vesicle and target membrane interactions.

Main Results:

  • SNARE proteins are essential regulators of vesicle transport from the Golgi complex to the cell surface in DCs.
  • The specific localization and binding partners of SNAREs provide insights into DC cytokine secretion pathways.
  • SNAREs play a critical role in orchestrating immune responses via DC cytokine secretion.

Conclusions:

  • SNARE proteins are key regulators of cytokine secretion in dendritic cells.
  • SNAREs offer valuable mechanistic insights into DC-mediated immune responses.
  • Targeting SNAREs in DCs presents a promising avenue for treating inflammatory diseases.