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Updated: Apr 14, 2026
Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
Published on: November 7, 2013
Ex vivo cytosolic delivery of functional macromolecules to immune cells
Armon Sharei1, Radiana Trifonova2, Siddharth Jhunjhunwala3
1Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America; The Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, United States of America; Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, United States of America; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
Abstract:
Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach's potential to engineer cell function is demonstrated in HIV infection studies.
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