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Surface enhanced resonance Raman detection of a catalytic intermediate of DyP-type peroxidase
Smilja Todorovic1, Peter Hildebrandt, Lígia O Martins
1Instituto de Tecnologia Química e Biológica (ITQB), Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal. smilja@itqb.unl.pt.
Physical Chemistry Chemical Physics : PCCP
|April 17, 2015
Abstract:
We report herein the vibrational spectroscopic characterisation of a catalytic intermediate formed by the reaction of H2O2 with DyP-type peroxidase immobilised on a biocompatible coated metal support. The SERR spectroscopic approach is of general applicability to other peroxidases which form relatively stable catalytic intermediates.
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