Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

47.1K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
47.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stepwise single-cell-resolved deep immunophenotyping pipeline to characterise immune heterogeneity and functionality in health and disease.

Journal of immunological methods·2026
Same author

PathoFact 2.0: an integrative pipeline for the prediction of antimicrobial resistance genes, virulence factors, toxins and toxin-associated proteins, and biosynthetic gene clusters in metagenomes.

GigaScience·2026
Same author

Integrative analysis of the mouse cecal microbiome across diet, age, and weight in the diverse BXD population.

Microbiome·2026
Same author

High-resolution multi-omics enhances prediction and detection of smORF-encoded proteins in the human gut microbiome.

Nature communications·2026
Same author

Freeze-Dried-Conserved Bacteria Can Be Identified by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Without the Need of Prior Culturing.

Biopreservation and biobanking·2026
Same author

Multi-meta-omics reveal distinct microbial genomic profiles and metabolic dysregulation in non-celiac gluten sensitivity.

mSphere·2026

Related Experiment Video

Updated: Apr 14, 2026

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

20.9K

Method optimization for fecal sample collection and fecal DNA extraction.

Conny Mathay1, Gael Hamot, Estelle Henry

  • 11 Integrated BioBank of Luxemburg (IBBL) , Luxembourg .

Biopreservation and Biobanking
|April 17, 2015
PubMed
Summary
This summary is machine-generated.

Optimized stool processing for DNA analysis ensures reliable results. The MSM I method with Genotek tubes provides superior DNA yield and quality for metagenomic studies.

More Related Videos

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples
07:24

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

Published on: December 10, 2014

15.1K
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

13.6K

Related Experiment Videos

Last Updated: Apr 14, 2026

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

20.9K
A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples
07:24

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

Published on: December 10, 2014

15.1K
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

13.6K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genomics

Background:

  • Formal method validation for biospecimen processing is crucial for laboratory and biobank accreditation.
  • This study focuses on optimizing a stool processing protocol for DNA-based analyses.

Purpose of the Study:

  • To optimize and validate a stool collection and processing protocol for downstream DNA metagenomic analysis.
  • To evaluate different DNA extraction methods and collection containers for optimal DNA yield and quality.

Main Methods:

  • Evaluated three DNA extraction methods (MSM I, Norgen Biotek, MoBio PowerMag) and six collection containers using human stool.
  • Assessed DNA quantity, quality, yield, purity, and reproducibility using spectrophotometry, spectrofluorometry, qPCR, SPUD assay, and 16S rRNA sequencing.

Main Results:

  • The optimal protocol uses 0.2g stool and 1000μL supernatant with the MSM I extraction method.
  • Genotek tubes (with stabilizer, RT storage) combined with MSM I extraction yielded optimal DNA quantity, quality, and reproducibility.
  • No inhibitors were detected at 25ng/μL, and the protocol demonstrated high reproducibility.

Conclusions:

  • A validated stool collection and processing method (MSM I with Genotek tubes) is suitable for DNA metagenomic analysis.
  • This method offers logistical advantages, including potential automation, and ensures high-quality DNA yield.
  • Laboratories and biobanks should systematically record protocol conditions for accreditation purposes.