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Updated: Apr 14, 2026
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Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
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[Dentin barrier cytotoxicity test with three-dimensional cell cultures]
1Dental Medical Devices Testing Center, Dental Materials Laboratory, National Engineering Laboratory for Digital and Material Technology of Stomatology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Summary
The dentin barrier test, using 3D cell cultures, shows lower cytotoxicity for dental materials compared to the filter diffusion test. This method better reflects clinical situations for evaluating dental material safety.
Area of Science:
- Dental Materials Science
- Biocompatibility Testing
- Cell Biology
Background:
- Cytotoxicity assessment is crucial for dental materials.
- Conventional filter diffusion tests may overestimate material toxicity.
- A more clinically relevant in vitro model is needed.
Purpose of the Study:
- To evaluate the cytotoxicity of dental filling materials and adhesives using a dentin barrier test.
- To compare these results with a conventional filter diffusion test.
- To determine the advantages of the dentin barrier test.
Main Methods:
- Six materials (Eugenol cement, zinc phosphate cement, glass ionomer cement, composite resin, REMI BOND, Adper Easy One) were tested.
- A dentin barrier test utilized 3D cultured L929 mouse fibroblasts and human dentin disks.
- Cytotoxicity was assessed via MTT assay, with results compared to a filter diffusion test.
Main Results:
- The dentin barrier test showed lower cytotoxicity for all tested materials compared to the filter diffusion test.
- Eugenol cement, REMI BOND, and Adper Easy One reduced cell survival to 82%, 63%, and 54% respectively in the dentin barrier test.
- Filter diffusion tests indicated moderate to severe cytotoxicity for most materials.
Conclusions:
- The dentin barrier test provides a more accurate assessment of dental material cytotoxicity.
- This 3D cell culture model correlates better with clinical scenarios.
- The dentin barrier test is advantageous for evaluating material biocompatibility.
