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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

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As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Related Experiment Video

Updated: Apr 14, 2026

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos
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Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos

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Long noncoding RNA CCAT1 promotes hepatocellular carcinoma progression by functioning as let-7 sponge.

Liang Deng1, Shi-Bin Yang2, Feng-Feng Xu3

  • 1Department of Hepatobiliary Surgery, The Eastern Hospital of the First Affiliated Hospital, Sun Yat-sen University, Eastern Huangpu Road No. 183, Guangzhou, 510700, China. dengliang201000@126.com.

Journal of Experimental & Clinical Cancer Research : CR
|April 18, 2015
PubMed
Summary

Long noncoding RNA CCAT1 is upregulated in hepatocellular carcinoma (HCC), promoting tumor growth and metastasis. CCAT1 acts as a sponge for let-7, impacting cancer progression and suggesting potential therapeutic applications.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are implicated in cancer biology.
  • CCAT1 is a lncRNA whose role in hepatocellular carcinoma (HCC) requires elucidation.

Purpose of the Study:

  • To investigate the expression, roles, and functional mechanisms of CCAT1 in HCC progression.

Main Methods:

  • Real-time PCR to quantify CCAT1 expression in HCC tissues.
  • In vitro assays to assess CCAT1's impact on cell proliferation and migration.
  • RNA immunoprecipitation and luciferase reporter assays to confirm CCAT1-miRNA interactions.

Main Results:

  • CCAT1 expression is significantly elevated in HCC tissues.
  • Increased CCAT1 correlates with larger tumor size, microvascular invasion, AFP levels, and poorer prognosis.
  • CCAT1 promotes HCC cell proliferation and migration by sponging let-7, leading to de-repression of HMGA2 and c-Myc.

Conclusions:

  • CCAT1 plays a critical role in HCC progression.
  • CCAT1 functions as a let-7 sponge, influencing HCC development.
  • CCAT1 presents a potential biomarker for HCC prognosis and a therapeutic target.