Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

GxP-Ready Single-Cell RNA-seq and Spatial Transcriptomics End-to-End Pipeline for Clinical Research.

Computational and structural biotechnology journal·2026
Same author

Durum Wheat cv. Svevo Reference Genome Rel.2.0: A Comprehensive Tool for Wheat Genomics.

Plant biotechnology journal·2026
Same author

Pyruvate carboxylase is critical for biofilm formation in Mycobacterium tuberculosis.

NPJ biofilms and microbiomes·2026
Same author

Development of a multipurpose diagnostic marker using PCR, real-time PCR, and LAMP assays for rapid detection of <i>Rhizoctonia solani</i> in rice plants and soil.

Frontiers in plant science·2026
Same author

Genomics for next-generation wheat breeding.

The plant genome·2026
Same author

Extensive variation between chromosomes of North American and European hop.

Nature communications·2026

Related Experiment Video

Updated: Apr 14, 2026

Laser-assisted Microdissection LAM as a Tool for Transcriptional Profiling of Individual Cell Types
09:31

Laser-assisted Microdissection LAM as a Tool for Transcriptional Profiling of Individual Cell Types

Published on: May 10, 2016

9.8K

Comparative transcriptome analysis within the Lolium/Festuca species complex reveals high sequence conservation.

Adrian Czaban1, Sapna Sharma2, Stephen L Byrne3

  • 1Department of Molecular Biology and Genetics, Aarhus University, Forsøgsvej 1, Slagelse, 4200, Denmark. Adrian.Czaban@mbg.au.dk.

BMC Genomics
|April 18, 2015
PubMed
Summary

Transcriptome analysis of the Lolium-Festuca complex reveals conserved gene families and identifies VRN2 as a key gene for vernalization. This research aids understanding of phenotypic diversity and potential genome transferability in important agricultural grasses.

More Related Videos

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
07:18

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

8.0K
Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

10.1K

Related Experiment Videos

Last Updated: Apr 14, 2026

Laser-assisted Microdissection LAM as a Tool for Transcriptional Profiling of Individual Cell Types
09:31

Laser-assisted Microdissection LAM as a Tool for Transcriptional Profiling of Individual Cell Types

Published on: May 10, 2016

9.8K
Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
07:18

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

8.0K
Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

10.1K

Area of Science:

  • Plant genomics
  • Transcriptomics
  • Evolutionary biology

Background:

  • The Lolium-Festuca complex, part of the agriculturally vital Pooideae subfamily, exhibits significant phenotypic diversity.
  • Understanding the molecular basis of this diversity is crucial for crop improvement.

Purpose of the Study:

  • To perform de novo transcriptome assemblies for four Lolium-Festuca species.
  • To identify candidate genes associated with phenotypic variations, such as vernalization.
  • To analyze gene family evolution and identify proteins under positive selection.

Main Methods:

  • De novo transcriptome assembly and protein prediction for four species.
  • Ortholog clustering using OrthoMCL and phylogenetic analysis.
  • Analysis of gene family conservation and evolutionary rates (dN/dS ratios).

Main Results:

  • Generated transcriptome assemblies with 52,166–72,133 transcripts per species.
  • Identified VRN2 as a candidate gene differentiating vernalization and non-vernalization types.
  • Discovered conserved gene families (91.61% identity) and identified proteins under positive selection potentially explaining stress tolerance differences.

Conclusions:

  • Comprehensive transcriptome comparison within the Lolium-Festuca complex.
  • VRN2 identified as a key gene for phenotypical differences.
  • High gene family conservation suggests genome knowledge transferability across species.