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[AS-PCR amplification in identification of Sedum sarmentosum with its relative species]
Objective:
To develop a scientific and effective method for identification of Sedum sarmentosum and its relative materials easily confused.
Methods:
DNA templates were extracted from plants, and DNA fragments of cpDNA trnL (tRNA-Leu) gene and trnL-trnF intergenic spacer were amplified and sequenced subsequently. trnL-trnF sequences of all samples were aligned.
Results:
The allele specific PCR method was studied with the designed allele-specific for distinguishing different samples. The result indicated that 300 bp and 500 bp DNA fragments were amplified from Sedum sarmentosum, where no any fragment was amplified from its relative species under the same reaction condition.
Conclusion:
The primers designed in this study are specific for Sedum sarmentosum from its relative species.
