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Updated: Apr 14, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
Published on: July 8, 2025
A label-free fluorescent molecular switch for a DNA hybridization assay utilizing a G-quadruplex-selective auramine O
Huiying Xu1, Fenghua Geng, Yongxiang Wang
1Department of Chemistry, State Key Lab of Molecular Engineering of Polymers and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China. bhliu@fudan.edu.cn.
Abstract:
A sensitive and selective assay of DNA is developed by utilizing a signal transduction strategy with the rational redesign of the hairpin structured G-quadruplex molecular switch (G4-MS) assembled using auramine O (AO). By monitoring the changes of the fluorescent signal, we could identify and further quantitatively determine the target DNA in the samples.

