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Low doses of hyperbaric oxygenation effectively decrease the size of necrotic zone in rats with experimental

E A Dotsenko¹, N V Nikulina, D P Salivonchik

¹Belarussian State Medical University, Minsk, the Republic of Belarus, ed_dots@mail.ru.

Bulletin of Experimental Biology and Medicine

|April 23, 2015

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Hyperbaric oxygenation reduced the risk zone in rat myocardium after coronary artery occlusion. A lower oxygen pressure (0.02 MPa) was more effective than 0.1 MPa in limiting infarct size.

Area of Science:

Cardiovascular Research
Hyperbaric Medicine
Myocardial Infarction

Background:

Myocardial infarction (MI) involves irreversible damage to heart muscle.
Assessing the impact of interventions on infarct size is crucial for treatment development.
Hyperbaric oxygenation (HBO) is explored for its therapeutic potential in ischemic conditions.

Purpose of the Study:

To investigate the effect of a single hyperbaric oxygenation session on infarct size in a rat model.
To compare the efficacy of different oxygen pressures (0.02 MPa and 0.1 MPa) during HBO therapy.
To analyze the impact of HBO on the risk, ischemic, and necrotic zones of the myocardium.

Main Methods:

Myocardial infarction was induced in rats by ligating the left coronary artery.

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Hyperbaric oxygenation (60-minute session) was administered 3 hours post-occlusion.

Planimetric evaluation was used to measure the size of risk, ischemic, and necrotic zones.

Main Results:

HBO significantly reduced the risk zone median from 31.7% to 25% of left ventricle weight.
At 0.02 MPa, HBO shifted the ischemia-to-necrosis ratio from 1.7:1 to 2:1, decreasing necrotic area.
At 0.1 MPa, HBO altered the ratio to 0.6:1, indicating a different effect on tissue zones.

Conclusions:

Hyperbaric oxygenation demonstrates a positive effect on myocardium during total coronary artery occlusion.
Lower oxygen pressure (0.02 MPa) during HBO therapy appears more beneficial for limiting infarct expansion.
HBO promotes a favorable redistribution of ischemic and necrotic areas, reducing overall infarct size.