Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Histamine Originating from the BNST Modulates Corticostriatal Synaptic Transmission during Early Postnatal Development.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same author

Monitoring brain chemistry: electrochemical techniques and applications.

Bioelectrochemistry (Amsterdam, Netherlands)·2026
Same author

Etiology and Outcomes of Pediatric Chest Pain in the ED: A Systematic Review.

Cureus·2026
Same author

Monitoring Molecules in Neuroscience 2024.

ACS chemical neuroscience·2026
Same author

Nanomaterial Modification of Ultramicroelectrodes Using Design-of-Experiments Principles.

ACS electrochemistry·2026
Same author

Pericardial Tamponade in Trauma: A Systematic Review of Diagnosis, Emergency Management, and Surgical Outcomes.

Cureus·2025

Related Experiment Video

Updated: Apr 14, 2026

In-depth Physiological Analysis of Defined Cell Populations in Acute Tissue Slices of the Mouse Vomeronasal Organ
10:11

In-depth Physiological Analysis of Defined Cell Populations in Acute Tissue Slices of the Mouse Vomeronasal Organ

Published on: September 10, 2016

8.2K

In vivo histamine voltammetry in the mouse premammillary nucleus.

Srimal Samaranayake1, Aya Abdalla, Rhiannon Robke

  • 1Department of Chemistry, Wayne State University, Detroit, USA. phashemi@chem.wayne.edu.

The Analyst
|April 24, 2015
PubMed
Summary

Researchers optimized an electrochemical method to detect histamine in the brain. This new technique allows for better understanding of histamine

More Related Videos

Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing
11:10

Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing

Published on: November 27, 2013

14.4K
Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry CIS-FSCV to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine
06:40

Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry CIS-FSCV to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine

Published on: April 23, 2020

3.8K

Related Experiment Videos

Last Updated: Apr 14, 2026

In-depth Physiological Analysis of Defined Cell Populations in Acute Tissue Slices of the Mouse Vomeronasal Organ
10:11

In-depth Physiological Analysis of Defined Cell Populations in Acute Tissue Slices of the Mouse Vomeronasal Organ

Published on: September 10, 2016

8.2K
Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing
11:10

Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing

Published on: November 27, 2013

14.4K
Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry CIS-FSCV to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine
06:40

Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry CIS-FSCV to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine

Published on: April 23, 2020

3.8K

Area of Science:

  • Neuroscience
  • Electrochemistry
  • Pharmacology

Background:

  • Histamine is a key mediator of allergic reactions and inflammation.
  • Histamine also functions as a neurotransmitter in the central nervous system, but its role is not well understood.
  • Understanding histamine neurotransmission is crucial for neurological disorder research.

Purpose of the Study:

  • To optimize an electrochemical waveform for selective histamine detection using fast scan cyclic voltammetry (FSCV).
  • To develop a novel in vivo model for studying histamine release in the brain.
  • To establish a robust method for identifying and characterizing in vivo histamine kinetics.

Main Methods:

  • Optimization of electrochemical waveforms for histamine detection in vitro.
  • Development of a physiological model involving stimulation of the medial forebrain bundle to release histamine in the mouse premammillary nucleus.
  • Application of fast scan cyclic voltammetry (FSCV) to detect and quantify histamine release in vivo.

Main Results:

  • A novel electrochemical waveform was developed, providing a unique, stimulation-locked signal for histamine.
  • A robust FSCV signal was achieved, enabling selective identification of histamine in complex biological media.
  • The study successfully characterized in vivo histamine kinetics in a mouse brain model.

Conclusions:

  • The optimized FSCV waveform and in vivo model provide a powerful tool for studying central nervous system histamine.
  • This advancement facilitates a deeper understanding of histamine's role in neurological functions and disorders.
  • The developed method enhances the ability to selectively detect and analyze histamine in real-time within the brain.