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Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing.

Yingnan Sun¹, Xiaodong Chen, Xiaoguang Zhou

¹State Key Laboratory of Integrated Optoelectronics, Institute of Semiconductors, Chinese Academy of Sciences, P.O. Box 912, Beijing 100083, PR China. yudeyu@semi.ac.cn.

|April 24, 2015

Summary

A new sequential inkjet printing method creates picoliter-scale multicomponent droplet arrays in oil. This technique enhances biochemical diagnostics and multistep screening experiments without external assistance.

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Area of Science:

Biochemical diagnostics
Microfluidics
Lab-on-a-chip technology

Background:

Inkjet printing is a novel method for fabricating microdroplet microarrays.
Existing inkjet printing technologies have limitations in general applicability for biochemical chip fabrication.

Purpose of the Study:

To introduce and verify a model for multiple injection procedures in inkjet printing.
To develop a sequential inkjet printing method for fabricating multicomponent droplet-in-oil arrays.

Main Methods:

Development of a multiple injection model for sequential inkjet printing.
Experimental validation of droplet fusion, rapid mixing, and multistep printing.
Application of piezoelectric inkjet printing technology for droplet array fabrication.

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Main Results:

Demonstration of a sequential inkjet printing method generating picoliter-scale multicomponent droplet-in-oil arrays.
Successful addressing of evaporation issues during picoliter droplet printing.
Validation of the method in dual fluorescence and enzyme inhibition assays.

Conclusions:

The sequential inkjet printing methodology enhances existing devices as universal diagnostic tools.
This technique accelerates the adoption of inkjet printing in multistep screening experiments.
The developed model improves the general applicability of inkjet printing for biochemical applications.