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Updated: Apr 14, 2026

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods
Published on: December 9, 2022
Experimental study on biodegradation of dairy wastewater using bacterial isolate
Livingston Peter Goldwyn1, M K Saseetharan, J Jeyanthi
1VLB Janakiammal Polytechnic College, Coimbatore - 641 042, India.
Bacillus sp. bacteria effectively degrade protein and chemical oxygen demand (COD) in dairy wastewater. A native strain achieved 92.8% protein removal and 91.3% COD removal, outperforming commercial inoculums for protein degradation.
Area of Science:
- Environmental Microbiology
- Wastewater Treatment Technologies
Background:
- Activated sludge processes utilize microorganisms for wastewater treatment.
- Bacillus sporogenes is a key bacterium in dairy wastewater.
- Dairy wastewater contains significant chemical oxygen demand (COD) and protein content.
Purpose of the Study:
- To investigate the efficiency of Bacillus sp. in removing COD and protein from dairy wastewater.
- To compare the biodegradation capabilities of a native Bacillus sp. strain with a commercial inoculum.
- To determine optimal cell concentrations for microbial degradation.
Main Methods:
- Isolation and inoculation of Bacillus sp. into dairy wastewater samples.
- Aerobic incubation with varying bacterial cell concentrations.
- Monitoring microbial growth via optical density.
- Quantification of COD and protein removal.
Main Results:
- Optimal protein degradation (92.8%) achieved in 14 days with 5 x 10^6 CFU/mL.
- Maximum COD removal (91.3%) observed in 18 days at the same cell concentration.
- Native strain showed higher protein removal efficiency compared to commercial inoculum.
- Commercial inoculum demonstrated higher COD removal efficiency.
Conclusions:
- Native Bacillus sp. strains are effective for dairy wastewater treatment, particularly for protein removal.
- Optimized inoculation strategies can enhance COD and protein biodegradation.
- Wastewater treatment can be improved by selecting appropriate native microbial strains or commercial inoculums based on target pollutant removal.
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