Modulation of protein synthesis by polyamines
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Updated: Apr 14, 2026

A Fluorescence-based Protocol for Preliminary Screening of Protein Synthesis Inhibitors from Natural Sources
Published on: January 27, 2026
Kazuei Igarashi1, Keiko Kashiwagi
1Department of Clinical Biochemistry, Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-Ku, Chiba, Japan; Amine Pharma Research Institute, Chuo-Ku, Chiba, Japan.
Abstract:
Polyamines are ubiquitous small basic molecules that play important roles in cell growth and viability. Since polyamines mainly exist as a polyamine-RNA complex, we looked for proteins whose synthesis is preferentially stimulated by polyamines at the level of translation, and thus far identified 17 proteins in Escherichia coli and 6 proteins in eukaryotes. The mechanisms of polyamine stimulation of synthesis of these proteins were investigated. In addition, the role of eIF5A, containing hypusine formed from spermidine, on protein synthesis is described. These results clearly indicate that polyamines and eIF5A contribute to cell growth and viability through modulation of protein synthesis.
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