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Related Concept Videos

Proteomics01:33

Proteomics

10.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Protein Networks02:26

Protein Networks

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An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
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Protein-protein Interfaces02:04

Protein-protein Interfaces

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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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Related Experiment Video

Updated: Apr 14, 2026

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
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Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

Published on: October 20, 2020

7.6K

Chemical proteomics of host-pathogen interaction.

Jingyan Ge1, Shao Q Yao1

  • 1Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.

Chemistry & Biology
|April 25, 2015
PubMed
Summary

Activity-based protein profiling (ABPP) is a key tool for studying molecular interactions. A new ABPP method identified the protein PheA as a potential effector in pathogen infections.

Area of Science:

  • Chemical Biology
  • Proteomics
  • Host-Pathogen Interactions

Background:

  • Activity-based protein profiling (ABPP) has become a standard technique for investigating small molecule-protein interactions within complex biological systems.
  • Understanding host-pathogen interactions is crucial for developing effective treatments against infectious diseases.

Purpose of the Study:

  • To introduce a novel ABPP method termed reactive probe-based chemical proteomics.
  • To apply this method to investigate host-pathogen interactions.
  • To identify key protein effectors involved in pathogen infection.

Main Methods:

  • Development and application of a novel reactive probe-based chemical proteomics strategy.
  • Utilizing ABPP to profile protein targets in a host-pathogen interaction model.

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Main Results:

  • Successful identification of the protein PheA as a potential key effector during pathogen infection.
  • Demonstration of the utility of the new ABPP method for host-pathogen studies.

Conclusions:

  • The novel reactive probe-based chemical proteomics approach is effective for studying host-pathogen interactions.
  • The identified protein PheA warrants further investigation as a potential therapeutic target in pathogen infections.