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A simple assay for glutathione in whole blood
Lovemore Hakuna1, Brandon Doughan, Jorge O Escobedo
1Department of Chemistry, Portland State University, Portland, OR 97201, USA. strongin@pdx.edu.
The Analyst
|April 28, 2015
Abstract:
A method for detecting glutathione selectively in whole blood deposited on filter paper is described. GSH is fractionated from proteins, hemoglobin and other potentially interfering components and determined using a resorufin-acrylate fluorescent probe. The relative standard deviation is lower than 5% (n = 5). Recoveries of GSH from whole blood are between 94% and 108.6%.

