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Related Experiment Video

Updated: Apr 14, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

G&T-seq: parallel sequencing of single-cell genomes and transcriptomes.

Iain C Macaulay¹, Wilfried Haerty², Parveen Kumar³

¹Sanger Institute-EBI Single-Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton, UK.

|April 28, 2015

Summary

We developed G&T-seq, a novel method for simultaneously sequencing single-cell DNA and RNA. This approach reveals cellular properties missed by analyzing DNA or RNA alone, advancing single-cell genomics research.

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Last Updated: Apr 14, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Area of Science:

Genomics
Molecular Biology
Cell Biology

Background:

Simultaneous analysis of genome and transcriptome in single cells is crucial for understanding gene regulation.
Existing methods face limitations in capturing both DNA and RNA information comprehensively.

Purpose of the Study:

To introduce and validate G&T-seq, a method for single-cell genome and transcriptome sequencing.
To demonstrate the utility of G&T-seq in uncovering novel cellular properties.

Main Methods:

G&T-seq protocol for separating and sequencing genomic DNA and full-length mRNA from individual cells.
Application of G&T-seq to over 220 single cells from mouse and human samples.

Main Results:

Successful simultaneous sequencing of genome and transcriptome from single cells.
Identification of cellular characteristics not discernible through DNA or RNA sequencing independently.
Demonstration of G&T-seq's capability in comprehensive single-cell analysis.

Conclusions:

G&T-seq provides a powerful tool for integrated genomic and transcriptomic analysis at the single-cell level.
This method enhances the understanding of genotype-phenotype relationships by linking genetic variation to gene expression.
G&T-seq opens new avenues for discovering cellular heterogeneity and function.