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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

101.9K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
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Genomics02:02

Genomics

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Genome Annotation and Assembly03:36

Genome Annotation and Assembly

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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Updated Human Chromosome Map of Lymphedema-lymphangiogenesis Genes: Template for Current and Future Discovery.

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Updated: Apr 14, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

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Next generation sequencing is here now.

R P Erickson

    Lymphology
    |April 29, 2015
    PubMed
    Summary
    This summary is machine-generated.

    Massively parallel DNA sequencing is affordable, but data analysis remains costly. Analyzing specific gene panels, like those for lymphedema, offers a cost-effective solution for genetic diagnostics.

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    Area of Science:

    • Genomics
    • Medical Genetics

    Background:

    • Massively parallel DNA sequencing technologies have significantly reduced gene sequencing costs.
    • The high cost of analyzing large-scale genomic data presents a challenge in clinical applications.

    Purpose of the Study:

    • To address the cost barrier in genetic analysis for patient conditions.
    • To introduce a cost-effective approach for genetic diagnostics.

    Main Methods:

    • Development of a targeted gene panel.
    • Focus on genes associated with lymphedematous conditions.

    Main Results:

    • A specific gene panel for lymphedema has been identified and described.
    • This approach makes genetic analysis more economically feasible.

    Conclusions:

    • Targeted gene panel analysis is a practical solution for managing the costs of genomic data interpretation.
    • This strategy facilitates the clinical application of genetic sequencing for specific diseases like lymphedema.