Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase II Reactions: Methylation Reactions01:17

Phase II Reactions: Methylation Reactions

904
Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...
904
Epigenetic Regulation01:37

Epigenetic Regulation

4.3K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
4.3K
Epigenetic Regulation01:46

Epigenetic Regulation

34.5K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
34.5K
Biosynthesis of Nucleic Acids01:28

Biosynthesis of Nucleic Acids

1.6K
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
1.6K
Mismatch Repair01:36

Mismatch Repair

45.8K
Overview
45.8K
Mismatch Repair01:20

Mismatch Repair

7.1K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
7.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Wnt4 as a signal coordinates DNA methylation and acetylation to determine germ line fate.

Developmental biology·2026
Same author

An Nlrp5-null mutation leads to attenuated de novo methylation in oocytes, accompanied by a significant reduction in DNMT3L.

Molecular human reproduction·2025
Same author

Roles and Regulation of DNA Methylation in Early Mammalian Development.

Annual review of animal biosciences·2025
Same author

The dominant follicle: the final frontier in bovine oocyte development.

Animal reproduction·2025
Same author

Deciphering differences in DNA methylation and transcriptome profiles of oocytes from pigs with high and low developmental competence.

Environmental epigenetics·2025
Same author

Combinatorial profiling of multiple histone modifications and transcriptome in single cells using scMTR-seq.

Science advances·2025
Same journal

Mutations in splicing factor gene U2AF1 rescue defective oncogene splicing in KRAS-mutant cancers.

Nature genetics·2026
Same journal

Assessing the effect of immune surveillance on clonal expansions in the blood.

Nature genetics·2026
Same journal

Improved heritability partitioning and enrichment analyses using summary statistics with graphREML.

Nature genetics·2026
Same journal

U2AF1 mutations rescue deleterious exon skipping induced by KRAS mutations.

Nature genetics·2026
Same journal

Lineage tracing from cellular heritage to disease destiny.

Nature genetics·2026
Same journal

Multiomics analysis of primary metabolism reveals the genetic basis of nitrogen partitioning modulated by ZmAVT1A-1 in maize.

Nature genetics·2026
See all related articles

Related Experiment Video

Updated: Apr 14, 2026

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

10.4K

Keeping methylation at bay.

Gavin Kelsey1

  • 1Epigenetics Programme, Babraham Institute, Cambridge, UK, and at the Centre for Trophoblast Research, University of Cambridge, Cambridge, UK.

Nature Genetics
|April 29, 2015
PubMed
Summary
This summary is machine-generated.

FBXL10 protein prevents DNA methylation of CpG islands, crucial for gene regulation and preventing disease. This finding is important for understanding developmental processes and leukemia.

More Related Videos

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

3.3K
Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina
07:50

Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina

Published on: August 29, 2018

9.6K

Related Experiment Videos

Last Updated: Apr 14, 2026

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

10.4K
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

3.3K
Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina
07:50

Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina

Published on: August 29, 2018

9.6K

Area of Science:

  • Genetics
  • Molecular Biology
  • Epigenetics

Background:

  • CpG islands are typically unmethylated, playing a key role in gene regulation.
  • Aberrant DNA methylation of CpG islands is linked to developmental disorders and diseases like leukemia.
  • Understanding mechanisms that maintain CpG island unmethylation is critical.

Purpose of the Study:

  • To investigate the role of FBXL10 in regulating DNA methylation.
  • To determine how FBXL10 influences the unmethylated state of CpG islands.
  • To elucidate the mechanism by which FBXL10 blocks CpG island methylation.

Main Methods:

  • The study likely involved molecular biology techniques to assess protein function and DNA methylation.
  • Investigated the interaction of FBXL10 with DNA methylation machinery.
  • Utilized genetic models or cell lines to study FBXL10's effect on CpG islands.

Main Results:

  • FBXL10 was identified as a component of a mechanism that actively blocks DNA methylation at CpG islands.
  • FBXL10 plays a role in maintaining the unmethylated state of these critical genomic regions.
  • Mutations in FBXL10, observed in leukemia, may disrupt this protective mechanism.

Conclusions:

  • FBXL10 is essential for preventing aberrant DNA methylation of CpG islands.
  • The function of FBXL10 is important for normal gene regulation during development.
  • Dysregulation of FBXL10 may contribute to the pathogenesis of diseases such as leukemia.