[Steadiness of multipotent mesenchymal stromal cells from adipose tissue to anoxia in vitro]

We have compared the effects of anoxia on multipotent mesenchymal stromal cells (MMSCs) preconditioned at 20 and 5 % O2. It has been shown that the lack of oxygen in the growth phase is accompanied by active cell proliferation, the decrease in their size and increasing the homogeneity of the population. At the same time, the anoxia reduced share of CD54-cells and did not change the number of cells expressing CD90 and CD73 surface markers. Preconditioning at 20% O2 increased the proliferative potential of cells, which nevertheless remained significantly, lower than that of the cells preconditioned at 5% O2. The level of lactate production was also significantly higher for cells preconditioned at 20% O2. However, the highest molar ration of lactate production and glucose consumption (Y(La/Glu)) was typical for MMSC pre-cultured at 5% O2. At anoxia, monolayer MMSCs conversely showed a decrease in this ratio by comparison with control cells and the cells cultured in growth phase. While maintaining a high level of viability under anoxia in the growth phase and in monolayer, we detected significant decrease in mitochondrial transmembrane potential. At the same time, the level of reactive oxygen species was increased only for MMSCs placed in anoxia in the active growth phase.